منابع مشابه
Immunological comparison of the starch branching enzymes from potato tubers and maize kernels.
Starch branching enzyme was purified from potato (Solanum tuberosum L.) tubers as a single species of 79 kilodaltons and specific antibodies were prepared against both the native enzyme and against the gel-purified, denatured enzyme. The activity of potato branching enzyme could only be neutralized by antinative potato branching enzyme, whereas both types of antibodies reacted with denatured po...
متن کاملStarch Branching Enzymes from Maize : Immunological Characterization using Polyclonal and Monoclonal Antibodies.
Spleen cells from mice immunized with starch branching enzymes were fused with cells from the mouse myeloma Sp2/0-AG14 cell line to form hybridomas. Those hybridomas producing antibodies against the branching enzyme were screened by the enzyme-linked immunosorbent assay using purified branching enzyme as the antigen. Three monoclonal cell lines (1A1D7, 1A1C3 and 4D2A9D8) were found to produce a...
متن کاملA cDNA encoding starch branching enzyme I from maize endosperm.
ADP-Glc pyrophosphorylase (EC 2.7.7.27), starch synthases (EC 2.4.1.21), and SBEs (EC 2.4.1.18) are the key enzymes in the pathway of plant starch biosynthesis. Starch is a polymer of Glc that exists as two fractions, amylose and amylopectin, in maize (Zea mays L.) kernel amyloplasts. The essentially linear polymer amylose contains a-1,4-linked Glc, whereas the branched polymer amylopectin cont...
متن کاملPhysical association of starch biosynthetic enzymes with starch granules of maize endosperm. Granule-associated forms of starch synthase I and starch branching enzyme II.
Antibodies were used to probe the degree of association of starch biosynthetic enzymes with starch granules isolated from maize (Zea mays) endosperm. Graded washings of the starch granule, followed by release of polypeptides by gelatinization in 2% sodium dodecyl sulfate, enables distinction between strongly and loosely adherent proteins. Mild aqueous washing of granules resulted in near-comple...
متن کاملStarch Branching Enzymes and their Genes in Sorghum
Mutisya, J.M. 2004. Starch branching enzymes and their genes in sorghum. Doctor’s dissertation. ISSN 1401-6249, ISBN 91-576-6768-3 Starch is an important raw material both for food and non-food purposes. It is synthesized and stored in source and sink tissues in plants. The starch deposited in amyloplasts of storage tissues possesses several physico-chemical properties, which makes it desirable...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1985
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.79.1.34